sipS [2019-07-10 16:47:03]

signal peptidase I

Locus: BSU23310

Isoelectric point: 9.72

Molecular weight: 20.90 kDa

Protein length: 184 aa

Gene length: 555 bp

Function: protein secretion

Product: signal peptidase I

Essential: no

E.C.: 3.4.21.89

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein secretion
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,432,316 → 2,432,870

Phenotypes of a mutant

• a sipS sipT double mutant is not viable PubMed

The protein

Catalyzed reaction/ biological activity

• cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins (according to Swiss-Prot)
• intra-membrane cleavage and thus inactivation of RsiV between A66 and M67 PubMed

Protein family

• peptidase S26 family (according to UniProt)

Paralogous protein(s)

• SipU, SipV, SipT

Structure

• 4NV4 (from Bacillus anthracis, 40% identity)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ypzJ-sipS
  Description: PubMed

• Genes: sipS
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• BKE23310 (ΔsipS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACTGATTTCCTCCAATAA, downstream forward: _UP4_TAGGATCAAGCAGCTTCCCA
• BKK23310 (ΔsipS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACTGATTTCCTCCAATAA, downstream forward: _UP4_TAGGATCAAGCAGCTTCCCA

Labs working on this gene/protein

• Jan Maarten van Dijl, Groningen, Netherlands

References

Reviews

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Original publications

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